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The sera of persons with strong allergic responses to the bites of the mosquito, Culex quinquefasciatus, contained IgE antibodies reactive with two major salivary gland proteins with molecular weights of 35 and 28 kDa. These antigens were purified, their amino termini sequenced, and the sequences were used to search for similar sequences in public databases. Two cDNAs, CuQu-D7Clu1 and CuQu-D7Clu12, which encode D7-related proteins, were identified as containing predicted amino acid sequences identical to the 35 and 28 kDa antigens, respectively. These proteins are expressed specifically in adult female salivary glands and, their predicted tertiary structures are consistent with a role as carriers of hydrophobic molecules in mosquito saliva.

Original publication

DOI

10.1016/s0965-1748(02)00168-6

Type

Journal article

Journal

Insect Biochem Mol Biol

Publication Date

01/2003

Volume

33

Pages

63 - 71

Keywords

Adolescent, Adult, Amino Acid Sequence, Animals, Antigens, Base Sequence, Blotting, Western, Child, Child, Preschool, Culex, DNA Primers, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, Humans, Immunoglobulin E, Molecular Sequence Data, Salivary Glands, Sequence Homology, Amino Acid